Journal: Developmental cell

Article Title: Macrophage-dependent cytoplasmic transfer during melanoma invasion in vivo

doi: 10.1016/j.devcel.2017.11.003

Figure Lengend Snippet: (A, B) Schematic of zebrafish hindbrain ventricle transplantation and imaging strategy. (C) Confocal Z-projection of fixed larvae in which melanoma cells (GFP immunostaining, green) are dorsal to the choroid plexus, within the skin (ZO-1 immunostaining, red). Scale bar is 20 uM. See also Figure S1 and Movie S1. (D) Left: DIC image of anterior region of 6dpf zebrafish larva on left. Right: Fluorescence image of same larva showing transplanted tumor (arrow) and disseminated cells (arrowheads). Scale bar is 100 uM. (E) Quantification of tumor cell dissemination in vivo in which each point is a single experiment comprising of 20–40 larvae. n indicates total number of zebrafish scored. Grey boxes indicate matched pairs of primary tumor-derived cell lines (A375P, WM793), and cell lines derived from metastases of those parental tumors (A375M1, 1205Lu). One-way ANOVA (p<0.0001), followed by non-parametric unpaired t-test (p=0.05 (between A375P and A375M1), p=0.01 (between WM793 and 1205Lu)).

Article Snippet: Human melanoma cells: A375M1 , ATCC , Cat#: CRL3222.

Techniques: Transplantation Assay, Imaging, Immunostaining, Fluorescence, In Vivo, Derivative Assay

Journal: Developmental cell

Article Title: Macrophage-dependent cytoplasmic transfer during melanoma invasion in vivo

doi: 10.1016/j.devcel.2017.11.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human melanoma cells: A375M1 , ATCC , Cat#: CRL3222.

Techniques: Virus, Recombinant, Blocking Assay, Red Blood Cell Lysis, Retroviral, Sequencing, Mutagenesis, Binding Assay, CRISPR, Plasmid Preparation, Software